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Citation and Date format for this article

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It appears that the established citation format for this article takes the form of...

[{{{URL}}} {{{WORK}}} {({{MMM DD, YYYY}}}) {{TITLE}}]

I don't agree that this is a good format, but it is the established one and should be taken into account when editing references. --User:Ceyockey (talk to me) 02:08, 24 February 2015 (UTC)[reply]

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Article update request

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— Preceding unsigned comment added by Christoper96 (talk • contribs)

Closing this because you haven't specified what you would like done. - MrOllie (talk) 16:43, 7 March 2023 (UTC)[reply]

Article update request

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Hi, Thanks a lot for your response. I would like to request you to update few things.

1) I would like to update this line from first paragraph of the article from "PacBio describes its platform as single-molecule real-time sequencing (SMRT), based on the properties of zero-mode waveguides." to the following: "PacBio works on two platforms. One is single-molecule real-time sequencing (SMRT), based on the properties of zero-mode waveguides and the second platform is the proprietary sequencing by binding (SBB) chemistry, which uses native nucleotides and scarless incorporation for DNA binding and extension.[1]

2) I would like to update the products section by adding this :"In October 2022, PacBio launched Revio system, which can sequence 1,300 human genomes per year as compared to 86 per year of the previous machines of PacBio."[2]

3) I would like to request to update the following line under "Reagents and SMRT Cells" from “Cells for the RS sequencer are slightly less than one-centimeter square and contain tens of thousands of zero-mode waveguides.” to this: "“Cells for the RS sequencer were slightly less than one-centimeter square and contained about 75,000 zero-mode waveguides (ZMWG), while each Cells for the Revio system contain 25 million ZMWGs.”[3][4]

Thanks a lot.

 Partly done For the first proposed change, can you provide an alternative reliable source that we can use for the information? The proposed reference appears to be a press release from the company, which are inherently promotional in nature with unclear fact-checking compared to other sources. A slightly modified version of the second proposed change was implemented. Can you provide a rationale for the third change? In my opinion, it seems to provide additional technical miscellany without clear explanation of what this means, and its unclear how the addition of that information would help better describe what the company does. To the contrary, it seems more of a sales type description of the company's products. I am marking this request as addressed; to re-open it, remove the "|A" in the {{request edit}} template above. SpencerT•C 22:27, 7 March 2023 (UTC)[reply]

Thank you for your response. Here is the reference for the first edit "Sequencing by Binding (SBB)":[5]

For the third edit, the company has upgraded it's DNA sequencing machinery that's why I would like to update the previous information. As per the directions, we can rewrite the line in neutral/non-promotional tone as following: "As of October 2022, each Cell of the new sequencing system by PacBio contains 25 million zero-mode waveguides." Reference 3 can be used as citation. Along with these references:[6][7]

Thank You very much.

Hi Christoper96, thanks for providing the additional information. I think we can add these to the article but have a few additional requests before that can happen. Can you provide an additional reference for the "single-molecule real-time sequencing" part? (First half of the sentence). Additionally, the cited article does not use the phrase "sequencing by binding", instead using "sequencing-by-synthesis (SBS)". Are these the same thing? If so, please propose an alternative form of the text based on the reference provided. Once done, this should be suitable for addition to the article.
For the second change, I like how the sentence is phrased, but would you be able to add an additional clause after it describing why this is impactful (e.g. "...contains 25 million zero-mode waveguides, allowing for sequencing of smaller sample volumes compared to prior systems" or something like that). I am also unable to view references 6 and 7, could you provide a quote of a sentence or two of relevant information so I can confirm the information? I was able to review the other references without issue. As before, please remove "|A" from the {{request edit}} template to re-open the request once the information requested has been provided. Best, SpencerT•C 22:01, 12 March 2023 (UTC)[reply]
Hi Spencer, thank you for your response.

1) Here is the reference for "single-molecule real-time sequencing": [8] You can kindly search "SBB" and you'll find sequencing by binding in reference 5.

2) Here is the rationale for the second change: "As of October 2022, each Cell of the new sequencing system by PacBio is denser and contains 25 million zero-mode waveguides (ZMWGs) as compared to each Cell of RS Sequencer, generating more data to speed up the DNA sequencing for large studies in human genetics." The References 6 and 7 are locked and require subscription.Here is the link to see the preview of the facts: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=pacbio+revio+25+million&btnG= Alternatively this reference can also be used:[9]

Thank you for your time.Regards

1.  Done My apologies for the oversight in missing that in reference 5. I have now made this change. I removed "proprietary" as that leans promotional, and the article mentioned another company also using an SBB platform.
2.  Done See how I phrased the proposed change; I made some tweaks but key content for the requested edit has been included. Best, SpencerT•C 22:41, 14 March 2023 (UTC)[reply]
Hi Spencer, thanks a lot for your time and co-operation. I would like to make just one last request. I would like to replace the image of an old product, “The Pacifiic Biosciences Sequel Sequencer” with “The PacBio Revio System”. You can choose any image from google search. https://www.google.com/search?q=revio+system+pacbio&client=firefox-b-d&source=lnms&tbm=isch&sa=X&ved=2ahUKEwiy1ZKOiN79AhW1mVYBHXtbAWcQ_AUoAXoECAEQAw&biw=1368&bih=797&dpr=2

Thank you very much for your time.

Hi, unfortunately images must comply with Wikipedia's image use policy, and I cannot chose images from a Google search to add to the article. If the company would be willing to release images under a free license to upload to Wikimedia commons, please follow the instructions here to see what may apply: [1]. SpencerT•C 22:36, 26 March 2023 (UTC)[reply]

References

  1. ^ "PacBio Announces Onso, the Highly Accurate Short-Read Sequencing Platform". Genomics Research from Technology Networks. Retrieved 7 March 2023.
  2. ^ Hale, Conor. "PacBio unveils its first short-read DNA sequencer plus a long-read system upgrade". Fierce Biotech. Retrieved 7 March 2023.
  3. ^ Levene, M. J.; Korlach, J.; Turner, S. W.; Foquet, M.; Craighead, H. G.; Webb, W. W. (31 January 2003). "Zero-Mode Waveguides for Single-Molecule Analysis at High Concentrations". Science. 299 (5607): 682–686. doi:10.1126/science.1079700. ISSN 0036-8075.
  4. ^ Hale, Conor. "PacBio unveils its first short-read DNA sequencer plus a long-read system upgrade". Fierce Biotech. Retrieved 7 March 2023.
  5. ^ Eisenstein, Michael (1 January 2023). "Illumina faces short-read rivals". Nature Biotechnology. 41 (1): 3–5. doi:10.1038/s41587-022-01632-4. ISSN 1546-1696. Retrieved 8 March 2023.
  6. ^ LeMieux, Julianna (1 December 2022). "PacBio Seeks to Maroon the Competition in the Crowded Sequencing Space". GEN Biotechnology. 1 (6): 476–478. doi:10.1089/genbio.2022.29066.jle. ISSN 2768-1572. Retrieved 8 March 2023.
  7. ^ Ansorge, W. J.; Katsila, T.; Patrinos, G. P. (1 January 2017). "Chapter 8 - Perspectives for Future DNA Sequencing Techniques and Applications". Molecular Diagnostics (Third Edition). Academic Press: 141–153. Retrieved 8 March 2023.
  8. ^ Rhoads, Anthony; Au, Kin Fai (1 October 2015). "PacBio Sequencing and Its Applications". Genomics, Proteomics & Bioinformatics. 13 (5): 278–289. doi:10.1016/j.gpb.2015.08.002. ISSN 1672-0229. Retrieved 13 March 2023.
  9. ^ Udine, Evan; Jain, Angita; van Blitterswijk, Marka (13 January 2023). "Advances in sequencing technologies for amyotrophic lateral sclerosis research". Molecular Neurodegeneration. p. 4. doi:10.1186/s13024-022-00593-1. Retrieved 13 March 2023.{{cite web}}: CS1 maint: unflagged free DOI (link)
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