Talk:Neurokinin B

This article was part of an assignment from Saint Louis University in Spring 2015 (see the course page for more details).

Copyright problem removed

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Gathering Sources for Initial Article Edits

I am beginning to compile a list of secondary sources to use during my research of Neurokinin B. Sources will continually be added as the article matures and an updated reference section will be maintained. These are the articles I have found relevant so far based on the outline I have created in my sandbox.

Please let me know of any additional sources that may be of use and if any of the sources I have presented have issues. Thanks!

• Role of Neurokinin B and Reproductive Neuroendocrinology
• Neurokinin B and the hypothalmic regulation of reproduction
• Current and Future applications of GnRH, Kisspeptin, and NKB analogues
• Tachykinin: Recent developments and novel roles in health and disease
• Neuropeptide co-expression in hypothalamic kisspeptin neurons of laboratory animals and the human
• Synthesis of Neurokinin B Analogs and Their Activities as Agonists and Antagonists

VarunS93 (talk) 21:37, 9 March 2015 (UTC)

References

VarunS93 (talk) 19:44, 22 February 2015 (UTC)

Ideas for Improvement

Since the article is of stub class there is not much to go off of. Good information added will result in improvement to the article. A link to my sandbox shows the outline that I am planning to add and follow to give the article about Neurokinin B more structure that what is currently presented.

If anyone has any additional ideas as to what I can look to add please let me know. My Goal is to make this article well rounded and helpful to those who are looking at it. VarunS93 (talk) 21:44, 9 March 2015 (UTC)

Peer Review

I think this article has a very strong foundation, and provides the reader with valuable links and a concise overview of NKB activity. As I continue with peer review assignment, I will separate my suggestions according to the article subtitles, and continue to evaluate additional subsections on subsequent days. This article is really nice and provides a lot of good information! Good work so far!

Suggestions

General

• I noticed that both "Neurokinin B" and "Neurokinin b" are used to refer to the peptide. I suggest either consistently using capital or lowercase "B".
• The link for reference 6 does not direct to a webpage
• Including a figure within one of the sections or subsections could be a nice addition to the article.

Introduction

• I would omit the "such as those of" in the second sentence because it seems kind of wordy. Perhaps say "Neurokinin B is implicated in a variety of human functions and pathways such as Gonadotropin-releasing hormone secretion activity.
• Maybe include the function of Kisspeptin by saying "Reproductive function is highly dependent on levels of both Neurokinin B and the G-protein coupled receptor ligand Kisspeptin." I also omitted the "not only" and said "both" to make it seem more inclusive grammatically.
• Instead of saying "The first instances of study done ..." I would reword this to "The first NKB studies attempted to resolve why high levels of the peptide may be implicated in pre-eclampsia during pregnancy."
• The last two sentences seem kind of wordy and redundant ("together"). Maybe say "NKB, Kisspeptin, and Dynorphin are found in the Arcuate (ARC) known as the KNDy subpopulation" This way the last sentence would seem less repetitive with the second use of "together"

Synthesis

• First sentence - replace "an end of chain" with "a terminal". I think this sounds more concise.
• Second sentence - Maybe omit "as follows". I don't know if that is necessary to say since the formula is immediately in the sentence.
• The last two sentences sound somewhat confusing, maybe I would say "In a later step, Preprotachykinin B is proteolytically cleaved into the pro-peptide proneurokinin B. This is followed by another cleavage from proneurokinin B, producing the final product, Neurokinin B."

Carolynslu (talk) 16:12, 31 March 2015 (UTC)

NKB in Humans

• Sentence 1 should be re-formatted. It might be somewhat confusing to the reader (especially towards the end of the sentence) : "During the Ovarian cycle GnRH secretion along with that of leutenizing hormone (LH) is highly regulated by a negative feedback system in with Neurokinin b along with its sister peptides the KNDy cell bodies play a role in."
• Sentence 2 : stimulate (there is an extra u)
• Sentence 3/4 might be able to condense into one sentence : Maybe say "In addition, studies have shown that the arcuate nucleus of females contains twice as many connection to NKB neurons than males; thus, indicating the peptide's increased role in female biological processes.

Receptors

• Sentence 2 - Instead of saying "in which tachykinin proteins bind," maybe say "that binds all tachykinin proteins."
• Sentence 3 - To be more concise, maybe say: "Neurokinin B has the ability to bind many Neurokinin receptors, with the highest affinity for the NK3R receptor group."
• Sentence 4 - Comma after "peptide"; also change "it binds to" to "that it binds"
• "In addition, Neurokinin b has also been found ..." Either omit "In addition" or "also" because using both is somewhat redundant

Role in the Release of Gonadotropin-Releasing Hormone

• Sentence 1 : Comma after "loop"; maybe change "...understimulated leading to..." to "...understimulated, which leads to..." (This may provide the sentence with a few more breaks)
• Sentence 2: Capitalization of K/kisspeptin, N/neurokinin B/b, D/dynorphin should be consistent ; also omit the extra "that"
• Sentence 3: Maybe say "to be conserved across..."
• Sentence 4: Comma after "groups"
• I would suggest slightly altering this sentence, as it somewhat sounds like an opinion. "These findings are important since GnRH release plays such a huge role in regulating hormonal control in the bodies of humans."

Figure

• The figure on the right hand side looks really good! I think this contributes to the integrity of Wikipedia Medical Articles

Carolynslu (talk) 16:36, 31 March 2015 (UTC)

Response

Thank you for your feedback, formatting and punctuation have always been a burden for me to accomplish in any kind of writing assignment. I appreciate you looking carefully through the article to find the mistakes that I made. I agree with all your suggestions and the changes in the article have been made to reflect that. I will also looking into trying to find a picture or two to add. I've had trouble finding something relevant but will keep searching.

Thank you again for your feedback! VarunS93 (talk) 14:50, 23 April 2015 (UTC)

Stub notation

• You can remove the notation at the bottom of the article page indicating the article is a stub. You've improved it beyond that now. I've made the change in the WikiProject boxes on this page already. Biolprof (talk) 02:06, 19 April 2015 (UTC)

Thank you! it has been removed.

VarunS93 (talk) 14:50, 23 April 2015 (UTC)

Second Peer Review

General

• I noticed two new subsections
  • Role in pre-eclampsia - Good addition. I think that it is important for Wikipedia medical articles to extend beyond topic facts by relating biological factors to prevalent diseases/conditions/complications
  • Studies in non-human animals - This is important to relate Neurokinin-B to active areas of research in the scientific community. It also bolsters the facts of your article by providing experimental studies that qualitatively/quantitatively evaluate the role of this specific gene.

Role in pre-eclampsia

• Maybe edit this sentence - As the severity of the disease can vary so can outcomes of mother and baby. In mild cases no harm usually comes of mother and baby. In more severe cases, constriction of blood vessels can result in an increased blood pressure and reduced blood flow.
  • I think that you can just say While mild forms of the disease do not significantly impact both mother and baby, more severe cases may lead to blood vessel constriction, increased blood pressure, and reduced blood flow
• Minor edits- Causes of pre-eclampsia have been widely researched, and the leading suspect in the investigation has always been the tachykinin peptide neurokinin B. During cases of pre-eclampsia insert comma placental expression of the TAC3 gene, which codes for NKB, was found in high levels.
• Sentence TAC3 in this case was able to secrete NKB in order to affect the circulation of the fetus. Additional studies done on rodents introduced to high levels of NKB indicated the vasoregulatory properties of the peptide, such as the vasoconstriction found in cases of pre-eclampsia
  • Maybe say - As a result, TAC-3 mediated NKB secretion affected fetal blood circulation. Additional rodent studies introducing high levels of NKB have identified vasoregulatory properties ....
• Minor edits In most cases of pre-eclampsia , the trophoblast was...
• Sentence In most cases NKB is called down to increase blood flow to the placenta in cases of defective implantation.
  • This sentence seems slightly redundant because it repeats "in most cases" from a previous sentence and within this sentence twice. Maybe investigate a different word choice, like In cases of defective implantation, NKB is vital to increase blood flow to the placenta or Pre-eclampsia typically involves the NKB-mediated increased placental blood flow during defective implantation
• This is somewhat unclear NKB usually found in the brain, has been found in the placenta at a concentration of 2.6 times that of the NKB in the brain.
• Overall - This section is really interesting! I think that it definitely improves that quality of the article by indicating the importance of NKB in fetal development. It is concise and descriptive for the medical audience, yet not too complicated for the everyday reader. I would just try to work on making this section a little bit more clear. I also like how this section includes non-human animal study results, which allows for a nice transition into the next subsection.

Studies in non-human animals

• Minor edit - Rodent studies have been performed and compared to human studies to determine the function of NKB.
• Sentence - In some cases, however, it is not possible to do experiments on humans and the rodent is substituted in due to the similarity of TAC3 and Tac2, NKB, and the TAC3R and Tac2r receptor genes.
  • Maybe say - For cases in which human studies are not possible, rodent studies are substituted due to the conserved similarity between TAC3 and Tac2, and NKB with the TAC3R and Tac2r receptor genes
• I would start the new paragraph here. This sentence seems out of place with what was previously said and may be better as an introduction to the next paragraph - Studies show that in postmenopausal woman there is an increased expression of tachykinin neurons in the arcuate nucleus.
• Maybe provide a handy Wikipedia link for ovariectomized so readers who do not have medical backgrounds can understand this term
• Minor edits - It was found that the ovariectomized rats presented a significant increase in the number of NKB neurons in the arcuate nucleus.[10]
• Overall - This section is interesting and important to compare rodent studies with human NKB biology. Are there any additional studies or comparisons that have been made between human and non-human NKB? Maybe another example would help bolster this section.

Carolynslu (talk) 16:54, 28 April 2015 (UTC)