Johannes F. Coy
Johannes F. Coy (born December 15, 1963, in Otzberg im Odenwald) is a German biologist and cancer researcher. He is the discoverer of the genes TKTL1[1] and DNaseX[2] (Apo10).[3] According to the latest findings in evolutionary research, TKTL1 is a key gene that has triggered increased neuron formation in the neocortex and structural improvements in the brain compared to Neanderthals, thus enabling the cognitive achievements of modern humans (homo sapiens).[4]
The evolutionary significance of TKTL1 was confirmed through studies by Nobel laureate Svante Pääbo and his research group at the Max Planck Institute for Evolutionary Anthropology. In collaboration with Wieland B. Huttner, they demonstrated that the TKTL1 gene, discovered by Johannes F. Coy, exhibits a single amino acid substitution in modern humans, leading to increased neurogenesis compared to Neanderthals.[4]
Life and scientific work
[edit]Johannes Coy began his biology studies at the Eberhard Karls University in Tübingen in 1985, which he completed in 1990 with a focus on molecular and human genetics as well as biochemistry. In the same year, he moved to the DKFZ in Heidelberg, where he became a member of the Molecular Genome Analysis research project headed by the then DKFZ director and later Nobel Prize winner for medicine, Prof. Harald zur Hausen, after completing his diploma thesis (mapping a tumor suppressor gene in neuroblastoma).
During this time, he concentrated on the identification of genes and discovered the two genes TKTL1 and DNaseX (Apo10) in this context. He was awarded summa cum laude in 1996 for his dissertation based on the discovery of these two genes. From his analyses of the TKTL1 and DNaseX (Apo10) genes, Coy concluded that both genes had the potential to be used as new diagnostic cancer markers.
In his further scientific work, Coy continued to focus on holistic research into tumor cell metabolism, in particular the use of the two genes for the early detection of cancer on the basis of diagnostic tests. He discovered that the simultaneous presence of TKTL1 and DNaseX (Apo10) in macrophages is indicative of cancer[3] and contributed to the development of a blood test that makes TKTL1 and DNaseX (Apo10) detectable in macrophages.[3]
He also discovered the TKTL1 metabolic pathway and the associated sugar metabolism that enables the prevention and repair of cell damage.[5][6][7]
Coy's diagnostic developments as a result of his research include:
- Epitope detection in monocytes (EDIM) - detection method of biomarkers in cells of the innate immune system in blood samples
- Automated flow cytrometry method
- Flow cytometry-based blood tests
Johannes Coy holds several patents in the field of cancer research and diagnostics, including DNaseX and TKTL1:
- DNA encoding DNase and related vectors, host cells and antibodies (DNaseX)[8]
- Transketolase-related protein (TKTL1)[9]
Awards
[edit]2007
[edit]- Waltraut Fryda Prize: Awarded at the International Congress for Biological Cancer Medicine for the elucidation of the role of the TKTL1 gene in the fermentation metabolism of cancer cells.
2006
[edit]- Diaita Science Prize: Awarded by the Society for Nutritional Medicine and Dietetics e.V. (now the Society for Nutritional Therapy and Prevention (FET) e.V.) at Medica for outstanding scientific commitment in the field of cancer research, diagnostics and therapy.
Publications (selection)
[edit]2022
[edit]- Targeted visualization based on blood tests enables early detection of premalignant and malignant tumors in asymptomatic individuals[10]
2017
[edit]- EDIM-TKTL1/Apo10 blood test: An innate immune system-based liquid biopsy for the early detection, characterization and targeted treatment of cancer[3]
2016
[edit]- A key role for transketolase-like 1 in metabolic reprogramming of tumors[5]
2013
[edit]- A biomarker-based detection and characterization of carcinomas using two fundamental biophysical mechanisms in mammalian cells[11]
2009
[edit]- Transketolase-like protein 1 (TKTL1) is required for rapid cell growth and full viability of human tumor cells[12]
2006
[edit]- Expression of the transketolase TKTL1 predicts survival of patients with colon and urothelial cancer. Reinterpretation of the Warburg effect[6]
2005
[edit]- Mutations in the transketolase-like gene TKTL1. Clinical implications for neurodegenerative diseases, diabetes and cancer[7]
2000
[edit]- Functional characterization of DNase X, a novel endonuclease expressed in muscle cells[13]
1996
[edit]- Molecular cloning of tissue-specific transcripts of a transketolase-related gene. Implications for the evolution of new vertebrate genes[1]
- Isolation, differential splicing and protein expression of a DNase on the human X chromosome[2]
References
[edit]- ^ a b Coy, Johannes F.; Dübel, Stefan; Kioschis, Petra; Thomas, Karen; Micklem, Gos; Delius, Hajo; Poustka, Annemarie (1996-03-15). "Molecular Cloning of Tissue-Specific Transcripts of a Transketolase-Related Gene: Implications for the Evolution of New Vertebrate Genes". Genomics. 32 (3): 309–316. doi:10.1006/geno.1996.0124. ISSN 0888-7543. PMID 8838793.
- ^ a b Coy, J. F.; Velhagen, I.; Himmele, R.; Delius, H.; Poustka, A.; Zentgraf, H. (April 1996). "Isolation, differential splicing and protein expression of a DNase on the human X chromosome". Cell Death and Differentiation. 3 (2): 199–206. ISSN 1350-9047. PMID 17180083.
- ^ a b c d Coy, Johannes F. (2017-04-20). "EDIM-TKTL1/Apo10 Blood Test: An Innate Immune System Based Liquid Biopsy for the Early Detection, Characterization and Targeted Treatment of Cancer". International Journal of Molecular Sciences. 18 (4): 878. doi:10.3390/ijms18040878. ISSN 1422-0067. PMC 5412459. PMID 28425973.
- ^ a b Pinson, Anneline; Xing, Lei; Namba, Takashi; Kalebic, Nereo; Peters, Jula; Oegema, Christina Eugster; Traikov, Sofia; Reppe, Katrin; Riesenberg, Stephan; Maricic, Tomislav; Derihaci, Razvan; Wimberger, Pauline; Pääbo, Svante; Huttner, Wieland B. (2022-09-09). "Human TKTL1 implies greater neurogenesis in frontal neocortex of modern humans than Neanderthals". Science. 377 (6611): eabl6422. doi:10.1126/science.abl6422. PMID 36074851.
- ^ a b Diaz-Moralli, Santiago; Aguilar, Esther; Marin, Silvia; Coy, Johannes F.; Dewerchin, Mieke; Antoniewicz, Maciek R.; Meca-Cortés, Oscar; Notebaert, Leen; Ghesquière, Bart; Eelen, Guy; Thomson, Timothy M.; Carmeliet, Peter; Cascante, Marta (2016-08-09). "A key role for transketolase-like 1 in tumor metabolic reprogramming". Oncotarget. 7 (32): 51875–51897. doi:10.18632/oncotarget.10429. ISSN 1949-2553. PMC 5239521. PMID 27391434.
- ^ a b Langbein, S.; Zerilli, M.; Zur Hausen, A.; Staiger, W.; Rensch-Boschert, K.; Lukan, N.; Popa, J.; Ternullo, M. P.; Steidler, A.; Weiss, C.; Grobholz, R.; Willeke, F.; Alken, P.; Stassi, G.; Schubert, P. (2006-02-27). "Expression of transketolase TKTL1 predicts colon and urothelial cancer patient survival: Warburg effect reinterpreted". British Journal of Cancer. 94 (4): 578–585. doi:10.1038/sj.bjc.6602962. ISSN 0007-0920. PMC 2361175. PMID 16465194.
- ^ a b Coy, Johannes F.; Dressler, Dirk; Wilde, Juergen; Schubert, Peter (2005). "Mutations in the transketolase-like gene TKTL1: clinical implications for neurodegenerative diseases, diabetes and cancer". Clinical Laboratory. 51 (5–6): 257–273. ISSN 1433-6510. PMID 15991799.
- ^ EP0842278B1, Zentgraf, Hanswalter; Poustka, Annemarie & Coy, Johannes et al., "Protein mit dnase-aktivität", issued 2005-11-09
- ^ EP0840789B1, Poustka, Annemarie & Coy, Johannes, "Transketolase-verwandtes protein", issued 2006-05-31
- ^ www.clinandmedimages.com http://web.archive.org/web/20230326080928/http://clinandmedimages.com/wp-content/uploads/2022/05/JCMI-v6-1541-1.pdf. Archived from the original (PDF) on 2023-03-26. Retrieved 2025-02-27.
{{cite web}}: Missing or empty|title=(help) - ^ Grimm, Martin; Schmitt, Steffen; Teriete, Peter; Biegner, Thorsten; Stenzl, Arnulf; Hennenlotter, Jörg; Muhs, Hans-Joachim; Munz, Adelheid; Nadtotschi, Tatjana; König, Klemens; Sänger, Jörg; Feyen, Oliver; Hofmann, Heiko; Reinert, Siegmar; Coy, Johannes F. (2013-12-04). "A biomarker based detection and characterization of carcinomas exploiting two fundamental biophysical mechanisms in mammalian cells". BMC Cancer. 13: 569. doi:10.1186/1471-2407-13-569. ISSN 1471-2407. PMC 4235042. PMID 24304513.
- ^ Xu, Xiaojun; zur Hausen, Axel; Coy, Johannes F.; Löchelt, Martin (2009). "Transketolase-like protein 1 (TKTL1) is required for rapid cell growth and full viability of human tumor cells". International Journal of Cancer. 124 (6): 1330–1337. doi:10.1002/ijc.24078. ISSN 1097-0215. PMID 19065656.
- ^ Los, Marek; Neubüser, Dagmar; Coy, Johannes F.; Mozoluk, Malgorzata; Poustka, Annemarie; Schulze-Osthoff, Klaus (2000-06-01). "Functional Characterization of DNase X, a Novel Endonuclease Expressed in Muscle Cells". Biochemistry. 39 (25): 7365–7373. doi:10.1021/bi000158w. ISSN 0006-2960. PMID 10858283.