Ribosylpyrimidine nucleosidase
| ribosylpyrimidine nucleosidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.2.2.8 | ||||||||
| CAS no. | 37288-60-1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, a ribosylpyrimidine nucleosidase (EC 3.2.2.8) is an enzyme that catalyzes the chemical reaction
- a pyrimidine nucleoside + H2O D-ribose + a pyrimidine base
Thus, the two substrates of this enzyme are pyrimidine nucleoside and H2O, whereas its two products are D-ribose and pyrimidine base.
This enzyme belongs to the family of hydrolases, specifically those glycosylases that hydrolyse N-glycosyl compounds. The systematic name of this enzyme class is pyrimidine-nucleoside ribohydrolase. Other names in common use include N-ribosylpyrimidine nucleosidase, pyrimidine nucleosidase, N-ribosylpyrimidine ribohydrolase, pyrimidine nucleoside hydrolase, RihB, YeiK, and nucleoside ribohydrolase. This enzyme participates in purine metabolism and pyrimidine metabolism.
Structural studies
[edit]As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1Q8F and 1YOE.
References
[edit]- Terada M, Tatibana M, Hayaishi O (1967). "Purification and properties of nucleoside hydrolase from Pseudomonas fluorescens". J. Biol. Chem. 242 (23): 5578–85. doi:10.1016/S0021-9258(18)99396-8. PMID 12325375.
- Petersen C, Moller LB (2001). "The RihA, RihB, and RihC ribonucleoside hydrolases of Escherichia coli. Substrate specificity, gene expression, and regulation". J. Biol. Chem. 276 (2): 884–94. doi:10.1074/jbc.M008300200. PMID 11027694.
- Giabbai B, Degano M (2004). "Cloning, purification, crystallization and X-ray analysis of the Escherichia coli pyrimidine nucleoside hydrolase YeiK". Acta Crystallogr. D. 60 (Pt 3): 524–7. doi:10.1107/S0907444903028488. PMID 14993681.
- Giabbai B, Degano M (2004). "Crystal structure to 1.7 a of the Escherichia coli pyrimidine nucleoside hydrolase YeiK, a novel candidate for cancer gene therapy". Structure. 12 (5): 739–49. doi:10.1016/j.str.2004.03.018. PMID 15130467.
