Resolution (chromatography)
Appearance
![](http://up.wiki.x.io/wikipedia/commons/thumb/5/5a/Chromatogram_in_English.svg/220px-Chromatogram_in_English.svg.png)
In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram.[1][2][3][4]
Expression
[edit]Chromatographic peak resolution is given by
where tR is the retention time and wb is the peak width at baseline. Here compound 1 elutes before compound 2.
If the peaks have the same width
- .
Plate number
[edit]![](http://up.wiki.x.io/wikipedia/commons/thumb/d/de/Rt_5_15.png/220px-Rt_5_15.png)
The theoretical plate height is given by
where L is the column length and N the number of theoretical plates.[5] The relation between plate number and peak width at the base is given by
- .
See also
[edit]References
[edit]- ^ IUPAC, Compendium of Chemical Terminology, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "Peak Resolution Rs in chromatography". doi:10.1351/goldbook.P04465
- ^ IUPAC, Compendium of Chemical Terminology, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "Peak Resolution in gas chromatography". doi:10.1351/goldbook.R05317
- ^ Maryutina, Tatiana A.; Savonina, Elena Yu.; Fedotov, Petr S.; Smith, Roger M.; Siren, Heli; Hibbert, D. Brynn (2018). "Terminology of separation methods (IUPAC Recommendations 2017)". Pure and Applied Chemistry. 90 (1): 181–231. doi:10.1515/pac-2017-0111. ISSN 0033-4545.
- ^ Ettre, L. S. (1993). "Nomenclature for chromatography (IUPAC Recommendations 1993)". Pure and Applied Chemistry. 65 (4): 819–872. doi:10.1351/pac199365040819. ISSN 1365-3075.
- ^ IUPAC, Compendium of Chemical Terminology, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "plate number, N". doi:10.1351/goldbook.P04694