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Light-oxygen-voltage-sensing domain

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A Light-oxygen-voltage-sensing domain (LOV domain) is a protein sensor used by a large variety of higher plants, microalgae, fungi and bacteria to sense environmental conditions. In higher plants, they are used to control phototropism, chloroplast relocation, and stomatal opening, whereas in fungal organisms, they are used for adjusting the circadian temporal organization of the cells to the daily and seasonal periods. They are a subset of PAS domains.[1]

Chromophore

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Common to all LOV proteins is the blue-light sensitive flavin chromophore, which in the signaling state is covalently linked to the protein core via an adjacent cysteine residue.[2][3] LOV domains are e.g. encountered in phototropins, which are blue-light-sensitive protein complexes regulating a great diversity of biological processes in higher plants as well as in micro-algae.[4][5][6][7] [8] Phototropins are composed of two LOV domains, each containing a non-covalently bound flavin mononucleotide (FMN) chromophore in its dark-state form, and a C-terminal Ser-Thr kinase.

Upon blue-light absorption, a covalent bond between the FMN chromophore and an adjacent reactive cysteine residue of the apo-protein is formed in the LOV2 domain. This subsequently mediates the activation of the kinase, which induces a signal in the organism through phototropin autophosphorylation.[9]

While the photochemical reactivity of the LOV2 domain has been found to be essential for the activation of the kinase, the in vivo functionality of the LOV1 domain within the protein complex still remains unclear.[10]

Fungus

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In case of the fungus Neurospora crassa, the circadian clock is controlled by two light-sensitive domains, known as the white-collar-complex (WCC) and the LOV domain vivid (VVD-LOV).[11][12][13] WCC is primarily responsible for the light-induced transcription on the control-gene frequency (FRQ) under day-light conditions, which drives the expression of VVD-LOV and governs the negative feedback loop onto the circadian clock.[13][14] By contrast, the role of VVD-LOV is mainly modulatory and does not directly affect FRQ.[12][15]

Natural and engineered functions of LOV domains

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LOV domains have been found to control gene expression through DNA binding and to be involved in redox-dependent regulation, like e.g. in the bacterium Rhodobacter sphaeroides.[16][17] Notably, LOV-based optogenetic tools [18] have been gaining wide popularity in recent years to control a myriad of cellular events, including cell motility,[19] subcellular organelle distribution,[20] formation of membrane contact sites,[21] microtubule dynamics,[22] transcription,[23] and protein degradation.[24]

See also

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References

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  1. ^ Edmunds, L. N. J. (1988). Cellular and Molecular Bases of Biological Clocks: Models and Mechanisms for Circadian Timekeeping. New York: Springer Verlag.
  2. ^ Peter, Emanuel; Dick, Bernhard; Baeurle, Stephan A. (2010). "Mechanism of signal transduction of the LOV2-Jα photosensor from Avena sativa". Nature Communications. 1 (8): 122. Bibcode:2010NatCo...1..122P. doi:10.1038/ncomms1121. PMID 21081920.
  3. ^ Peter, Emanuel; Dick, Bernhard; Baeurle, Stephan A. (2012). "A novel computer simulation method for simulating the multiscale transduction dynamics of signal proteins" (PDF). The Journal of Chemical Physics. 136 (12): 124112. Bibcode:2012JChPh.136l4112P. doi:10.1063/1.3697370. PMID 22462840.
  4. ^ Hegemann, P. (2008). "Algal sensory photoreceptors". Annual Review of Plant Biology. 59: 167–89. doi:10.1146/annurev.arplant.59.032607.092847. PMID 18444900.
  5. ^ Christie, J. M. (2007). "Phototropin blue-light receptors". Annual Review of Plant Biology. 58: 21–45. doi:10.1146/annurev.arplant.58.032806.103951. PMID 17067285.
  6. ^ Briggs, W. R. (2007). "The LOV domain: A chromophore module servicing multiple photoreceptors". Journal of Biomedical Science. 14 (4): 499–504. doi:10.1007/s11373-007-9162-6. PMID 17380429.
  7. ^ Kottke, Tilman; Hegemann, Peter; Dick, Bernhard; Heberle, Joachim (2006). "The photochemistry of the light-, oxygen-, and voltage-sensitive domains in the algal blue light receptor phot". Biopolymers. 82 (4): 373–8. doi:10.1002/bip.20510. PMID 16552739.
  8. ^ Veetil, S.K; Mittal, C; Ranjan, P; Kateriya, S (July 2011). "A conserved isoleucine in the LOV1 domain of a novel phototropin from the marine alga Ostreococcus tauri modulates the dark state recovery of the domain". Biochim Biophys Acta. 1810 (7): 675–82. doi:10.1016/j.bbagen.2011.04.008. PMID 21554927.
  9. ^ Jones, M. A.; Feeney, K. A.; Kelly, S. M.; Christie, J. M. (2007). "Mutational analysis of phototropin 1 provides insights into the mechanism underlying LOV2 signal transmission". Journal of Biological Chemistry. 282 (9): 6405–14. doi:10.1074/jbc.M605969200. PMID 17164248.
  10. ^ Matsuoka, D.; Tokutomi, S. (2005). "Blue light-regulated molecular switch of Ser/Thr kinase in phototropin". Proceedings of the National Academy of Sciences of the United States of America. 102 (37): 13337–42. Bibcode:2005PNAS..10213337M. doi:10.1073/pnas.0506402102. PMC 1198998. PMID 16150710.
  11. ^ Peter, Emanuel; Dick, Bernhard; Baeurle, Stephan A. (2012). "Illuminating the early signaling pathway of a fungal light-oxygen-voltage photoreceptor". Proteins: Structure, Function, and Bioinformatics. 80 (2): 471–481. doi:10.1002/prot.23213. PMID 22081493. S2CID 206406322.
  12. ^ a b Heintzen, C.; Loros, J. J.; Dunlap, J. C. (2001). "The PAS protein VIVID defines a clock-associated feedback loop that represses light input, modulates gating, and regulates clock resetting". Cell. 104 (3): 453–64. doi:10.1016/s0092-8674(01)00232-x. PMID 11239402. S2CID 18003169.
  13. ^ a b Lee, K.; Dunlap, J. C.; Loros, J. J. (2003). "Roles for WHITE COLLAR-1 in circadian and general photoperception in Neurospora crassa". Genetics. 163 (1): 103–14. doi:10.1093/genetics/163.1.103. PMC 1462414. PMID 12586700.
  14. ^ Gardner, G. F.; Feldman, J. F. (1980). "The frq locus in Neurospora crassa: A key element in circadian clock organization". Genetics. 96 (4): 877–86. doi:10.1093/genetics/96.4.877. PMC 1219306. PMID 6455327.
  15. ^ Hunt, S. M.; Thompson, S.; Elvin, M.; Heintzen, C. (2010). "VIVID interacts with the WHITE COLLAR complex and FREQUENCY-interacting RNA helicase to alter light and clock responses in Neurospora". Proceedings of the National Academy of Sciences of the United States of America. 107 (38): 16709–14. Bibcode:2010PNAS..10716709H. doi:10.1073/pnas.1009474107. PMC 2944716. PMID 20807745.
  16. ^ Conrad, Karen S.; Bilwes, Alexandrine M.; Crane, Brian R. (2013). "Light-Induced Subunit Dissociation by a Light–Oxygen–Voltage Domain Photoreceptor from Rhodobacter sphaeroides". Biochemistry. 52 (2): 378–91. doi:10.1021/bi3015373. PMC 3582384. PMID 23252338.
  17. ^ Metz, S.; Jager, A.; Klug, G. (2011). "Role of a short light, oxygen, voltage (LOV) domain protein in blue light- and singlet oxygen-dependent gene regulation in Rhodobacter sphaeroides". Microbiology. 158 (2): 368–379. doi:10.1099/mic.0.054700-0. PMID 22053008.
  18. ^ Wittmann T, Dema A, van Haren J (May 2020). "Lights, cytoskeleton, action: Optogenetic control of cell dynamics". Current Opinion in Cell Biology. 66. Elsevier Ltd.: 1–10. doi:10.1016/j.ceb.2020.03.003. PMC 7577957. PMID 32371345.
  19. ^ Wu, Yi I.; Frey, Daniel; Lungu, Oana I.; Jaehrig, Angelika; Schlichting, Ilme; Kuhlman, Brian; Hahn, Klaus M. (2009-01-01). "A genetically encoded photoactivatable Rac controls the motility of living cells". Nature. 461 (7260): 104–8. Bibcode:2009Natur.461..104W. doi:10.1038/nature08241. PMC 2766670. PMID 19693014.
  20. ^ van Bergeijk, Petra; Adrian, Max; Hoogenraad, Casper C.; Kapitein, Lukas C. (2015-01-01). "Optogenetic control of organelle transport and positioning". Nature. 518 (7537): 111–4. Bibcode:2015Natur.518..111V. doi:10.1038/nature14128. PMC 5063096. PMID 25561173.
  21. ^ Jing, Ji; He, Lian; Sun, Aomin; Quintana, Ariel; Ding, Yuehe; Ma, Guolin; Tan, Peng; Liang, Xiaowen; Zheng, Xiaolu (2015-01-01). "Proteomic mapping of ER–PM junctions identifies STIMATE as a regulator of Ca2+ influx". Nature Cell Biology. 17 (10): 1339–47. doi:10.1038/ncb3234. PMC 4589512. PMID 26322679.
  22. ^ van Haren J, Charafeddine RA, Ettinger A, Wang H, Hahn KM, Wittmann T (Mar 2018). "Local control of intracellular microtubule dynamics by EB1 photodissociation". Nature Cell Biology. 20 (3). Nature Research.: 252–261. doi:10.1038/s41556-017-0028-5. PMC 5826794. PMID 29379139.
  23. ^ Baaske, Julia; Gonschorek, Patrick; Engesser, Raphael; Dominguez-Monedero, Alazne; Raute, Katrin; Fischbach, Patrick; Müller, Konrad; Cachat, Elise; Schamel, Wolfgang W. A.; Minguet, Susana; Davies, Jamie A. (2018-10-09). "Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells". Scientific Reports. 8 (1): 15024. Bibcode:2018NatSR...815024B. doi:10.1038/s41598-018-32929-7. ISSN 2045-2322. PMC 6177421. PMID 30301909.
  24. ^ Renicke, Christian; Schuster, Daniel; Usherenko, Svetlana; Essen, Lars-Oliver; Taxis, Christof (2013). "A LOV2 Domain-Based Optogenetic Tool to Control Protein Degradation and Cellular Function". Chemistry & Biology. 20 (4): 619–626. doi:10.1016/j.chembiol.2013.03.005. ISSN 1074-5521. PMID 23601651.
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