Hygromycin B

Hygromycin B
Identifiers
	IUPAC name O-6-Amino-6-deoxy-L-glycero- D-galacto- heptopyranosylidene- (1-2-3)-O-beta-D-talopyranosyl (1-5) -2-deoxy-N3-methyl-D-streptamine;
CAS Number	31282-04-9;
PubChem CID	35766;
CompTox Dashboard (EPA)	DTXSID7041045 ;
ECHA InfoCard	100.045.935
Chemical and physical data
Formula	C20H37N3O13
Molar mass	527.53 g/mol (563.5 with HCl) g·mol−1

Hygromycin B, is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis^[1].

History

Hygromycin B was originally developed in the 1950's for use with animals and is still added into swine and chicken feed as an anthelmintic or anti-worming agent (product name: Hygromix). Hygromycin B is produced by Streptomyces hygroscopicus, a bacterium isolated in 1953 from a soil sample. Resistance genes were discovered in the early 1980's.^[2]^[3]

Use in research

In the laboratory it is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene. The resistance gene is a kinase that inactivates hygromycin B through phosphorylation.^[4] Since the discovery of hygromycin-resistance genes, hygromycin B has become a standard selection antibiotic in gene transfer experiments in many prokaryotic and eukaryotic cells.

External links

Information from hygromycin.net (InvivoGen)

mechanism of action: [1]
resistance genes: [2]
chemistry, stability: [3]

References

^ McGuire, Pettinger (1953), "Hygromycin I. Preliminary studies on the production and biological activity of a new antibiotic.", Antibiot. Chemother., 3: 1268–1278
^ Davies, Gritz (1983), "Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae.", Gene, 25: 179–88
^ Burgett, Kaster (1983), "Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing.", Nucleic Acids Res., 11: 6895–911
^ Rao RN, Allen NE, Hobbs JN, Alborn WE, Kirst HA, Paschal JW (1983). "Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli". Antimicrobial Agents and Chemotherapy. 24 (5): 689–95. PMID 6318654.{{cite journal}}: CS1 maint: multiple names: authors list (link)

[discovery-1] McGuire, Pettinger (1953), "Hygromycin I. Preliminary studies on the production and biological activity of a new antibiotic.", Antibiot. Chemother., 3: 1268–1278

[res1-2] Davies, Gritz (1983), "Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae.", Gene, 25: 179–88

[res2-3] Burgett, Kaster (1983), "Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing.", Nucleic Acids Res., 11: 6895–911

[pmid6318654-4] Rao RN, Allen NE, Hobbs JN, Alborn WE, Kirst HA, Paschal JW (1983). "Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli". Antimicrobial Agents and Chemotherapy. 24 (5): 689–95. PMID 6318654.{{cite journal}}: CS1 maint: multiple names: authors list (link)

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