Jump to content

File:Tissue processing - Tissue sections on slides are stained on an automated stainer.jpg

Page contents not supported in other languages.
This is a file from the Wikimedia Commons
From Wikipedia, the free encyclopedia

Original file (2,848 × 4,288 pixels, file size: 5.21 MB, MIME type: image/jpeg)

Description

Tissue sections on slides are stained using hematoxylin and eosin on an automated stainer. This is the routine "H&E" stain, which is used on almost all specimens. Hematoxylin, which is blue or purple, stains mostly nuclei, while the pink to orange eosin stains cytoplasm, nucleoli, and extracellular substances like collagen.

After staining, the processor takes the slides through a dehydrating solvent, usually alcohol or acetone, and then into a "clearing agent" either xylene or a citrus-smelling substance called Americlear.

Sometimes it is necessary to supplement the H&E with "special stains" or "immunostains"
Date
Source originally posted to Flickr as Tissue Processing 12.jpg
Author Ed Uthman
Permission
(Reusing this file)
This image, which was originally posted to Flickr, was uploaded to Commons using Flickr upload bot on 8 November 2008, 12:13 by Jacopo Werther. On that date, it was confirmed to be licensed under the terms of the license indicated.
w:en:Creative Commons
attribution share alike
This file is licensed under the Creative Commons Attribution-Share Alike 2.0 Generic license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • share alike – If you remix, transform, or build upon the material, you must distribute your contributions under the same or compatible license as the original.

Captions

Add a one-line explanation of what this file represents

Items portrayed in this file

depicts

20 October 2006

0.016666666666667 second

32 millimetre

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current12:33, 8 November 2008Thumbnail for version as of 12:33, 8 November 20082,848 × 4,288 (5.21 MB)Flickr upload botUploaded from http://flickr.com/photo/78147607@N00/275494796 using Flickr upload bot

The following 2 pages use this file:

Global file usage

The following other wikis use this file:

Metadata