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File:BASIC Parts Preparation.png

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Summary

Description
English: The DNA parts are synthesised with integrated prefix and suffix sequences (iP and iS) that contain BsaI sites. Restriction digest with BsaI is followed by incubation with the prepared iP and iS linkers. These linkers anneal via the complementary 4bp overhang and are ligated onto the DNA part. The parts are thus ready to be used for BASIC assembly.
Date
Source Own work
Author Lee Yun Jie

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Preparation of BASIC Parts

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17 April 2020

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current03:50, 17 April 2020Thumbnail for version as of 03:50, 17 April 20202,647 × 1,768 (159 KB)Scarecrow399Text edit
03:49, 17 April 2020Thumbnail for version as of 03:49, 17 April 20202,647 × 1,768 (157 KB)Scarecrow399Uploaded own work with UploadWizard

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